​Department of Physics “G. Occhialini”

This is a set of images taken on In2Sight devices coupled to PVC (polyvinylchloiride), implanted in mice. -- Animal implants. Each mouse (BALB/cAnNHsd, Envigo) received one In2Sight device, which was implanted subcutaneously in the inner part of the hind leg. Mice were anesthetized intraperitoneally with 100mg/kg ketamine and 4mg/kg xylazine. The operating field was prepared by hair trichotomy followed by appropriate disinfection with 70° ethanol or betadine. Two incisions were then made using a sterile scalpel. After positioning the device, a tight suture (single stitch) of the incision was performed using a sterile 5/0 absorbable suture thread. For the first 2 days, pain control was foreseen by administering opioids (Buprenorphine hydrochloride 0.16mg/kg subcutaneously) and infections avoided by administering an antibiotic (Enrofloxacin 5 mg/kg for 5 days). All the implants were done under the animal permit n. 551/2023-PR of the Italian ministry for health. The device, once coupled with acrylic glue to a slab of PVC (Polyvynil chloride, 5 mm in diameter, 0.3 mm thickness), was sterilized by STERRAD before the implant that lasted up to 6 weeks. The mice were kept in a pathogen‐free conventional animal house facility. The animal house is run by professional employees fully equipped with state‐of‐the‐art instrumentation in order to maintain the standard of animal welfare at the maximum levels. All mice were housed in individual, ventilated cages with 12 h light/dark cycles with food and water ad libitum. The mice were euthanized at the end of the implant by cervical dislocation right before the imaging session. Experiments were performed using protocols approved by the Institutional Animal Care and Use Committee of the University of Milano‐Bicocca and by the Italian Ministry of Health. -- Chemicals. Hoechst 33342 (Sigma-Aldrich Chemical Company (Steinheim, Germany)) was used to stain nuclei in ex-vivo experiments. -- Multiphoton imaging. Third harmonic generation and 3-photon excitation fluorescence were selected by the Semrock filters FF02-447/60 and FF562-Di03-25, respectively. For SHG the exc. wavelength was 800 nm (emission 400/40 nm). λexc was 770 nm in case of Hoechst nuclei staining (485/30 nm). Auto-fluorescence in the green channel was collected at 535/50 nm. FILE NAME FORMAT for folder TPE_SHG_6weeks: name@800P140mWBP400BP535z200um50piani_Ch2 is taken as an example. name: if "lente#" (#=1,..4) means one of the 4 lenses on top of the microatlas; if "spacer" means the In2Sight spacers; if "fralenti" means the space between the lenses and therefore in the free tissue 800P140m: excitation wavelength = 800 nm, average power = 140 mW, WBP400BP535z200um50piani: means emission filters at 400 nm (Ch1) and 535 nm (Ch2); 200 micrometer full depth of stack, 50 layers. FORMAT in THG_3PE_data_1week: modality, exc. , through the lenses or control, FOV in um, image size in pixels, the depth, the number of averages (#avg).

This archive contains the example datasets, implementations of the rational splines and P-spline methods, along with code to reproduce the benchmark and the generate the figures in the publication "Shape preserving interpolation with arbitrary smoothness based on mollification". Archive structure ----------------- - `interp.py`: implementation of the interpolation methods - `benchmark.py`: program that benchmarks the interpolation code - `plots.py`: program that generates the figures of the article - `proofs.py`: mathematical proofs in [SymPy][1] of some results used to implement the rational splines - `shell.nix`: [Nix][2] expression that builds a reproducible environment to run the programs

Image taken under SHG and THG at 1300 nm on IN2SIGHT devices coupled to PVC (Poly-vinyl-chloride) slabs implanted in mice. These data have been analyzed and used for the Ms. submitted on June 2026.

This dataset contains experimental data used in the manuscript: Electrochemical Impedance Spectroscopy as a tool for diagnosing reactive species in plasma-treated water. The dataset includes electrochemical impedance spectroscopy (EIS) measurements, calibration data for NaCl, HNO3 solutions and Plasma treated water, plasma discharge power data, and chemical properties of plasma-treated water (pH, concentration of NO2-, NO3- by multimeter). EIS measurements were performed in the frequency range 0.1 Hz to 10 kHz using an MFIA impedance analyzer. Plasma-treated water was prepared under different discharge currents and treatment times. Sheets included in the dataset, 1-Impedance data for plasma-treated water, 2- Calibration data for NaCl solutions at different concentrations, 3- Calibration data for HNO3 acid solutions, 4-Electrical parameters of plasma discharge, 5-Impedance parameters (fmax, Im(Y), Gm, Q, alpha) for all samples in two different setups, 6- Chemical measurements including pH, concentration of NO2- by Griess assay and NO3- measured by multimeter.

This data set contains the data collected under the IN2SIGHT EU project (GA. 964481) for the test of different types of microlenses used for the project. These data refer to the year 2023. The microlenses have been tested with a USAF1951 target to evaluate the OSF and the MTF and have been used in imaging when implanted or on cells. A specific set of data regards the use of agarose phantoms containing fluorescent microbeads to evaluate the PSF in 3D. Each folder contains README.txt files and a "summary.pptx" graphical summary. The full list of folders' tree can be found in the file Implants_MetaFile_2023.xlsx The file "Metafile_Ulenses_2023.xlsx" contains the description of the folders tree with comments. The same file in the tab README file reports the type of microlenses investigated in vitro and in vivo. Details on the work done can be found in the deliverables of WP2 and WP4 of the project available at the EU web site https://cordis.europa.eu/project/id/964481/reporting.

Dataset for the article Multimodal comparison of cold atmospheric plasma sources for biomedical applications

A search for $W\gamma$ resonances in the mass range between 0.7 and 6.0 TeV is presented. The $W$ boson is reconstructed via its hadronic decays, with the final-state products forming a single large-radius jet, owing to a high Lorentz boost of the $W$ boson. The search is based on proton-proton collision data at $\sqrt{s} = 13 ~\text{TeV}$, corresponding to an integrated luminosity of 137 $\text{fb}^{-1}$, collected with the CMS detector at the LHC in 2016--2018. The $W\gamma$ mass spectrum is parameterized with a smoothly falling background function and examined for the presence of resonance-like signals. No significant excess above the predicted background is observed. Model-specific upper limits at 95% confidence level on the product of the cross section and branching fraction to the $W\gamma$ channel are set. Limits for narrow resonances and for resonances with an intrinsic width equal to 5% of their mass, for spin-0 and spin-1 hypotheses, range between 0.17 fb at 6.0 TeV and 55 fb at 0.7 TeV. These are the most restrictive limits to date on the existence of such resonances. In specific narrow-resonance benchmark models, heavy scalar (vector) triplet resonances with masses between 0.75 (1.15) and 1.40 (1.36) TeV are excluded for a range of model parameters. Model-independent limits on the product of the cross section, signal acceptance, and branching fraction to the $W\gamma$ channel are set for minimum $W\gamma$ mass thresholds between 1.5 and 8.0 TeV.

This is the second part of the dataset contains the data used for the publication Conci, L. Sironi, E. Jacchetti, D. Panzeri, D. Inverso, R. Martinez-Vazquez, R. Osellame, M. Collini, G. Cerullo, G. Chirico, and M.T. Raimondi, “In vivo label-free tissue histology through a microstructured imaging window. ,” APL Bioeng. accepted november 2023. The detailed description of the contents can be found in the file "Dataset_chickenEmbyo.docx", readable with Word or equivalent. In brief, the whole dataset contains 6 folders, each with a set of data taken either on histological sections, stained with H&E, or in vivo in chicken Embryos with fluorescence optical microscopy, either confocal or two-photon excitation. Two folders are in the part 1 of this data set. The remaining 4 folders can be found here.

This is the first part of the dataset contains the data used for the publication Conci, L. Sironi, E. Jacchetti, D. Panzeri, D. Inverso, R. Martinez-Vazquez, R. Osellame, M. Collini, G. Cerullo, G. Chirico, and M.T. Raimondi, “In vivo label-free tissue histology through a microstructured imaging window. ,” APL Bioeng. accepted november 2023. The detailed description of the contents can be found in the file "Dataset_chickenEmbyo.docx", readable with Word or equivalent. In brief, the dataset contains 6 folders, each with a set of data taken either on histological sections, stained with H&E, or in vivo in chicken Embryos with fluorescence optical microscopy, either confocal or two-photon excitation. The first two folders can be found here. The remaining 4 folders are in the second part of the dataset.

This dataset contains a set of data related to the characterization of the 2-photon polymerized microstructures for the IN2SIGHT project. Mostly, they are supplementary data for publications.