University of Milano-Bicocca Showcase

20S proteasome inhibitors have been approved for the treatment of multiple myeloma but induce chemotherapy-related neurotoxicity. Here, we compared the neurotoxicity of bortezomib (BTZ) and carfilzomib (CFZ), a less neurotoxic drug, by investigating preclinical models and dissecting the underlying molecular mechanisms using a multidimensional approach. We developed a new mouse model of CFZ-induced neuropathy and compared it to an established BTZ model using behavioral, morphological/morphometric, and proteomic analyses showing that the more severe neurotoxicity correlated with loss of nerve fibers and protein expression changes. Moreover, mitotoxicity and cytoskeleton alterations were comparatively investigated in terms of onset of altered mitochondrial morphology, functionality, and trafficking, alongside cytoskeletal proteins expression and axonal degeneration in cultured mouse dorsal root ganglion neurons. Both compounds significantly altered mitochondrial network organization and energy production after 24 hours of treatment. However, only BTZ increased microtubule hyper-stabilization by accumulation of tubulin post-translational modifications and induced early axonal degeneration within the first 10 hours, severely impacting mitochondrial trafficking after 24 hours. Taken together, these results point at mitochondrial toxicity as a common downstream effect of both treatments, while BTZ-specific off-target activity on tubulin hyper-stability may initiate early mitochondrial trafficking alterations, a new knowledge helping to inform future mitigation approaches. Data refer to each figure in the article describing the in vivo and in vitro effects of the treatment with proteasome inhibitors Bortezomib and Carfilzomib.

Metabolite libraries built from NMR spectra for two types of coffee (green or roasted) and for three extraction procedures (hydroalcoholic, espresso, moka), using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 12.0 software.

A new synthetic, multi-purpose dataset - called ENRICH - for testing photogrammetric and computer vision algorithms. Compared to existing datasets, ENRICH offers higher resolution images also rendered with different lighting conditions, camera orientation, scales, and field of view. Specifically, ENRICH is composed of three sub-datasets: ENRICH-Aerial, ENRICH-Square, and ENRICH-Statue, each exhibiting different characteristics. The proposed dataset is useful for several photogrammetry and computer vision-related tasks, such as the evaluation of hand-crafted and deep learning-based local features, effects of ground control points (GCPs) configuration on the 3D accuracy, and monocular depth estimation. Each zip file in the root is relative to a specific dataset: - ENRICH-Aerial, is an aerial image block of the city of Launceston, Australia. The acquisition is performed by simulating a typical oblique aerial camera with five views (nadir and four oblique views). - ENRICH-Square, is a ground-level dataset of a square captured by four cameras, each one moving on a different path with different focal length, orientation, and lighting conditions. - ENRICH-Statue, is a ground-level dataset portraying a statue (placed in the center of the ENRICH-Square scene), acquired using four cameras moving on different paths with different focal lengths, orientations, and lighting conditions. Be sure to check the README file in the dataset root for information on folder structure and file contents. Please refer to the related paper (https://doi.org/10.1016/j.isprsjprs.2023.03.002) for information about the generation method and the purpose of ENRICH.

Microplastic abundance in 6 supraglacial debris samples collected on On 20 July 2018 and 24 July 2018 on the Forni Glacier (Italian Alps, approximate coordinates of sampling location 46°12’30’’ N, 10°13’50’’ E, altitude 2580 m a.s.l.).

ODMR data for a single microcrystal

Background: Antibody validation for tissue staining is required for reproducibility and criteria to ensure validity have been published recently. The majority of these recommendations imply the use of routinely processed tissue (FFPE). Materials & Methods: We applied to lightly fixed frozen sections a panel of 126 antibodies validated for FFPE with extended criteria. Results: Less than 30% performed conservatively with all fixations, 35% preferred one fixation over another, 13% gave non-specific staining, 23% did not stain at all. Conclusions: Individual antibody variability of the paratope fitness for the fixed antigen may be the cause. Re-validation of established antibody panels is required when applied to sections whose fixation and processing is different from the tissue where they have been initially validated. These are supplementary Data to the manuscript. In addition Tabel 1 and Supplementary Table 1 are provided in Excel format.

Supplementary materials, neuroimaging data, and analysis code for the submitted manuscript: Unaware Processing of Words Activates Experience-Derived Information in Conceptual-Semantic Brain Networks Marta Ghio1,2,*, Barbara Cassone3,*, Marco Tettamanti2,3 1 Faculty of Mathematics and Natural Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany; 2 CIMeC - Center for Mind/Brain Sciences, University of Trento, Italy; 3 Department of Psychology, University of Milan-Bicocca, Milano, Italy. * Equal contribution. Keywords: conceptual-semantic representations; experience; awareness; continuous flash suppression; manipulable objects; emotions.

Dataset and materials for the rTMS study "Keeping order in the brains: the supramarginal gyrus and serial order in short-term memory". In the "Experiment folders" there are the E-Prime script of the tasks and the stimuli used. In the "Database and all anlysis" folder, there is the anonymus excel database of all 4 experiments and the Statistica workbook with all the analyses conducted and reported in the paper.

This set of communication tools is indicated for understanding the needs of locked-in (LIS) patients unable to speak or communicate through verbal, sign, or body language, and is ideal for communicating through Brain Computer Interface (with the P300 paradigm, speller, machine learning, EEG/ERP classification, eye tracking). It comprises 60 validated, easily comprehensible, pictorial tables depicting adult persons in various contexts that affect their physiological or psychological state. Their motivational states are illustrated in a small cloud. The drawings are in color and representative of both sexes and various ethnicity. THIS MATERIAL CAN BE FREELY USED FOR RESEARCH OR CLINICAL PURPOSES, PROVIDED THAT APPROPRIATE CREDIT IS GIVEN TO THE SOURCE. Proverbio, A.M., Pischedda, F. (2022). Validation of a Pictionary-based communication tool for assessing individual needs and motivational states in locked-in patients (P.A.I.N. set). Terms and conditions: This material cannot be used for commercial purposes. Therefore it cannot be transferred to technological devices of any type and be sold. This material cannot be placed in any internet web site nor can it be provided to profit making companies, or to the media (television, journals). To request the PAIN set for clinical use, or for non-profit academic research purposes, write to mado.proverbio@unimib.itWe do not accept requests directly from students, nor do we accept any requests from students on behalf of their advisor. Students should ask their faculty advisor to make the request.

The Adula unit (Central Alps) exposes high- and ultra-high-pressure (HP-UHP) metamorphic rocks, providing key insights into the subduction and exhumation dynamics of the former distal European margin. In the San Bernardino pass area (Switzerland) it is possible to reconstruct the tectonometamorphic evolution or the Adula unit from HP conditions to exhumation and subsequent shearing during orogen-parallel extension. The P-T-d-t evolution of eclogites and host rocks fhas been determined by thermodynamic modelling and Ar-Ar dating of syntectonic minerals. This dataset contains location and description of studied samples, mineral chemistry data obtaine by electron microprobe analyses and Ar-Ar data on white micas in eclogites and hosting orthogneisses.