​Department of Biotechnology and Biosciences

Metabolite libraries built from NMR spectra for the metabolomics analysis of cinnamon extracts, using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 14.1 software.

Structure of bombesin peptide calculated in presence of 150 mM SDS micelles at pH 7.0, 25°C. Structure was determinded by NMR spectroscopy and SA-MD. Data clearly show that, under these experimental conditions, bombesin adopts a α-helix structure.

Metabolite libraries built from NMR spectra for natural, lavado and commercial cocoa and for two extraction procedures (hydroalcoholic and Soxhlet), using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 12.4 software.

The .diam files are the creter diameters to be used with the software craterstats2 The olimpo_nocart.exe is the executable to calculate the water splash; input data are requested when running the code; the txt files should not be modified The runup_water_acheron6_pss is a small fortran code to calculate the run-up and velocity of the water wave with the analytical formula provided in the text

Metabolite library built from NMR spectra for metabolomic analysis of leaves' hydroalcholic extract (80% MeOH) from wild mountain Mentha longifolia, using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 14.1 software.

Metabolite library built from NMR spectra for metabolomic analysis of leaves' aquous extract from Lolium multiflorum, using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 14.1 software.

Metabolite libraries built from NMR spectra for two types of coffee (green or roasted) and for three extraction procedures (hydroalcoholic, espresso, moka), using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 12.0 software.

Revision of Hydatigera taeniaeformis species complex with a description of a new species (Lavikainen et al., IJP 2016): 1. Morphological data. A drawing (atypical segment). Morphological matrix. 2. DNA data. Nucleotide sequence alignments (18S rDNA; pold & pepck; mitochondrial protein-coding genes; cox1 complete haplotype data set).

This record contains raw data related to the article “Modeling Cardiomyopathies in a Dish: State-of-the-Art and Novel Perspectives on hiPSC-Derived Cardiomyocytes Maturation". Abstract The stem cell technology and the induced pluripotent stem cells (iPSCs) production represent an excellent alternative tool to study cardiomyopathies, which overcome the limitations associated with primary cardiomyocytes (CMs) access and manipulation. CMs from human iPSCs (hiPSC-CMs) are genetically identical to patient primary cells of origin, with the main electrophysiological and mechanical features of CMs. The key issue to be solved is to achieve a degree of structural and functional maturity typical of adult CMs. In this perspective, we will focus on the main differences between fetal-like hiPSC-CMs and adult CMs. A viewpoint is given on the different approaches used to improve hiPSC-CMs maturity, spanning from long-term culture to complex engineered heart tissue. Further, we outline limitations and future developments needed in cardiomyopathy disease modeling.

Abstract Background Microbial lipids have been emerging as a sustainable alternative to vegetable oils and animal fat to produce biodiesel and industrial relevant chemicals. The use of wastes for microbial processes can represent a way for upgrading low value feedstock to high value products, addressing one of the main goals of circular economy, the reduction of wastes by recycling. Two oleaginous yeasts, Rhodosporidiobolus azoricus and Cutaneotrichosporon oleaginosum, were used in this study to demonstrate the feasibility of the proposed approach. Results In this study wastes from industrial food processing, as pumpkin peels and syrup from candied fruits manufacture, were used for yeast cultivation and for lipids production. Evaluation of growth and sugar consumption revealed marked differences between the yeasts in capacity to utilize the main sugars present in the feedstock. In particular, we observed an unexpected limitation in glucose metabolism on mineral defined media by R. azoricus. Both species showed ability to grow and accumulate lipids on media exclusively composed by undiluted pumpkin peel hydrolysate, and R. azoricus was the best performing. By a two-stage process carried out in bioreactor, this species reached a biomass concentration of 45 g/L (dry weight) containing 55% of lipids, corresponding to a lipid concentration of 24 g/L, with a productivity of 0.26 g/L/h and yield of 0.24 g lipids per g of utilized sugar. Conclusions Wastes from industrial food processing were sufficient to completely support yeast growth and to induce lipid accumulation. This study provides strong evidence that the concept of valorisation through the production of lipids from the metabolism of nutrients present in agro-industrial wastes by oleaginous yeasts is promising for implementation of biotechnological processes in a circular economy contest.