Rejuvenated vintage tissue sections highlight individual antigen fate during processing and long term storage.

Published: 31 August 2021| Version 1 | DOI: 10.17632/z65mr2yfc7.1
Contributors:
,
,
,

Description

Antigen-bearing proteins become progressively unavailable to immunodetection after prolonged storage of routine sections, exposed to a variety of agents, such as moisture, oxygen and temperature. By proteomic analysis, the antigens are retained in the sections and definitely in the tissue block, pointing to fixation-independent, storage time-dependent protein modifications. Based on previous experience, we hypothesized that a combined exposure to a reducing agent and to chemicals favouring protein conformation changes would reverse the masking in aged sections. Disaccharides, lactose and sucrose, and a surfactant, added to a standard antigen retrieval buffer, reverse the negative changes in aged sections. Furthermore, they provide enhanced access to antigens in freshly cut sections, but not universally, revealing additional factors, besides heat and calcium chelation, required for antigen retrieval of individual proteins. This dataset contains supplementary data to the manuscript, raw data and analytical tools.

Files

Institutions

Universita degli Studi di Milano-Bicocca

Departments

School of Medicine and Surgery

Categories

Pathology, Immunoassay, Immunochemistry, Immunohistochemistry, Histopathology, Histochemistry

License