Skip to main content
Bicocca Open Archive Research Data

Datasets within this collection

Filter Results
404 resultsSearch results powered by
  • Rejuvenated vintage tissue sections highlight individual antigen fate during processing and long term storage.
    Antigen-bearing proteins become progressively unavailable to immunodetection after prolonged storage of routine sections, exposed to a variety of agents, such as moisture, oxygen and temperature. By proteomic analysis, the antigens are retained in the sections and definitely in the tissue block, pointing to fixation-independent, storage time-dependent protein modifications. Based on previous experience, we hypothesized that a combined exposure to a reducing agent and to chemicals favouring protein conformation changes would reverse the masking in aged sections. Disaccharides, lactose and sucrose, and a surfactant, added to a standard antigen retrieval buffer, reverse the negative changes in aged sections. Furthermore, they provide enhanced access to antigens in freshly cut sections, but not universally, revealing additional factors, besides heat and calcium chelation, required for antigen retrieval of individual proteins. This dataset contains supplementary data to the manuscript, raw data and analytical tools.
    • Software/Code
    • Tabular Data
  • Cannabinoids: an effective treatment for chemotherapy-induced peripheral neurotoxicity?
    materials and methods as supplementary data to the review "Cannabinoids: an effective treatment for chemotherapy-induced peripheral neurotoxicity?"
    • Dataset
    • Document
  • Data for: Relative Prognostic Importance of Left and Right Ventricular Ejection Fraction in Patients with Cardiac Diseases
    3D echocardiography volumes and ejection fraction of the left and right ventricle in patients with various cardiac diseaes who were followed for 3.7 years after the echo study to assess outcome
    • Tabular Data
    • Dataset
  • Supplementary tables/figures and referecences to A Prospective Evaluation of Health Care Provider and Patient Assessments in Chemotherapy Induced Peripheral Neurotoxicity.
    Supplementary Tables and Figures are made available here.
    • Dataset
    • Document
  • SARS-CoV-2 CodonUsage Data
    • Dataset
  • Human Intravenous Immunoglobulin Alleviates Neuropathic Symptoms in a Rat Model of Paclitaxel-Induced Peripheral Neurotoxicity
    This record contains raw data related to the original article entitled "Human Intravenous Immunoglobulin Alleviates Neuropathic Symptoms in a Rat Model of Paclitaxel-Induced Peripheral Neurotoxicity".
    • Dataset
  • Antibodies validated for routinely processed tissue unpredictably stain frozen tissue sections.
    Background: Antibody validation for tissue staining is required for reproducibility and criteria to ensure validity have been published recently. The majority of these recommendations imply the use of routinely processed tissue (FFPE). Materials & Methods: We applied to lightly fixed frozen sections a panel of 126 antibodies validated for FFPE with extended criteria. Results: Less than 30% performed conservatively with all fixations, 35% preferred one fixation over another, 13% gave non-specific staining, 23% did not stain at all. Conclusions: Individual antibody variability of the paratope fitness for the fixed antigen may be the cause. Re-validation of established antibody panels is required when applied to sections whose fixation and processing is different from the tissue where they have been initially validated. These are supplementary Data to the manuscript. In addition Tabel 1 and Supplementary Table 1 are provided in Excel format.
    • Tabular Data
    • Dataset
    • File Set
  • Data for: TOPIRAMATE PREVENTS OXALIPLATIN-RELATED AXONAL HYPEREXCITABILITY AND OXALIPLATIN INDUCED PERIPHERAL NEUROTOXICITY.
    raw data
    • Software/Code
    • Dataset
  • The immune landscape of uterine leiomyosarcomas
    Personalized immune intervention to release and redirect the T cells against a tumor has shown substantial progress in aggressive tumors such as melanoma and lung cancer, despite the fact that predictors of sustained response are still unclear. Data on less common histotypes are scanty. Among soft tissue sarcomas, uterine leiomyosarcomas (ULMS) have a dire prognosis, yet therapeutic advances are needed in order to improve the actual treatment. Immune checkpoint inhibitor therapy has been applied to exceptionally few cases, of which the immune cell composition was not examined in detail. We analyzed in situ the inflammatory infiltrate of 21 untreated ULMS in high-dimensional, single cell phenotyping on routinely processed tissue, directed at the characterization of lymphoid cells and macrophages. T-lymphoid cells displayed a composite phenotype common to all tumors, suggestive of antigen-exposure; in about half of the cases containing sufficient lymphocytes, we found evidence of exhaustion and a CD8+ TCF7+ phenotype, this latter associated with T-cell reactivation. To the contrary, myelomonocytic cells had case-specific individual combinations of phenotypes and subsets. We identified five distinct monocyte-macrophage cell types: histiocytes, phagocytes, tumor-associated macrophages, inflammatory monocytes and myelomonocytic cells of undefined phenotype. Immunosuppressive molecules (TIM3, B7H3, VISTA, PD1, PDL1) were heterogeneously expressed in inflammatory and endothelial cells. The heterogeneity and phenotype of the monocyte-macrophage population may represents a challenge for which we provide an initial understanding.
    • Other
    • Software/Code
    • Image
    • Tabular Data
    • Dataset
    • File Set
  • Keeping order in the brain: the supramarginal gyrus and serial order in short-term memory - dataset and materials
    Dataset and materials for the rTMS study "Keeping order in the brains: the supramarginal gyrus and serial order in short-term memory". In the "Experiment folders" there are the E-Prime script of the tasks and the stimuli used. In the "Database and all anlysis" folder, there is the anonymus excel database of all 4 experiments and the Statistica workbook with all the analyses conducted and reported in the paper.
    • Other
    • Image
    • Tabular Data
    • Dataset
    • Audio
1