​School of Medicine and Surgery

Final scoring sheet for the cases enrolled in the study

Dataset archives

Here a section of a human papillary thyroid carcinoma, analysed with MALDI-MSI and H&E stained. The dataset includes: -H&E stained WSI (ptc_core_tma_q3.tif); -ROIs (geojson); SCiLS exchange format ROIs (SEF.zip); -imzML of the differnt ROIs (imzML.zip). The ROIs were obtained using the workflow presented in "Improving the annotation for Spatial Proteomics: A computational approach to enhance molecular characterization of Thyroid Nodules" . A detailed description and scripts can be found at https://github.com/Vsc0/msi-enhanced .

Normal human lymph nodes and tonsils, multiplexed with the MILAN technique, analyzed with BRAQUE.

20S proteasome inhibitors have been approved for the treatment of multiple myeloma but induce chemotherapy-related neurotoxicity. Here, we compared the neurotoxicity of bortezomib (BTZ) and carfilzomib (CFZ), a less neurotoxic drug, by investigating preclinical models and dissecting the underlying molecular mechanisms using a multidimensional approach. We developed a new mouse model of CFZ-induced neuropathy and compared it to an established BTZ model using behavioral, morphological/morphometric, and proteomic analyses showing that the more severe neurotoxicity correlated with loss of nerve fibers and protein expression changes. Moreover, mitotoxicity and cytoskeleton alterations were comparatively investigated in terms of onset of altered mitochondrial morphology, functionality, and trafficking, alongside cytoskeletal proteins expression and axonal degeneration in cultured mouse dorsal root ganglion neurons. Both compounds significantly altered mitochondrial network organization and energy production after 24 hours of treatment. However, only BTZ increased microtubule hyper-stabilization by accumulation of tubulin post-translational modifications and induced early axonal degeneration within the first 10 hours, severely impacting mitochondrial trafficking after 24 hours. Taken together, these results point at mitochondrial toxicity as a common downstream effect of both treatments, while BTZ-specific off-target activity on tubulin hyper-stability may initiate early mitochondrial trafficking alterations, a new knowledge helping to inform future mitigation approaches. Data refer to each figure in the article describing the in vivo and in vitro effects of the treatment with proteasome inhibitors Bortezomib and Carfilzomib.

Data refer to the contribute of cytokines and macrophages in two chronic models of chemotherapy-induced peripheral neuropathy (CIPN) comparing paclitaxel (PTX)- and oxaliplatin (OHP)-induced CIPN in rats. Results consists in behavioural, morphological/ morphometrical and neurophysiological assessments, obtained with the administration of PTX 10 mg/kg once a week and OHP 5 mg/kg twice a week.

Results of the pre and post-QuANTUM survey to a panel of 12 international digital and computational pathologists for the assessment of absolute and % tumor cellularity.

Supplementary data for the manuscript "Seeing or believing in hyperplexed spatial proteomics via antibodies." The content of this online repository is explained in the .txt file "FolderContent-ReadMe". Please download and read.

Granular data from the assessment of non-inferiority of different monitors/scanners combination for the evaluation of whole slide images (WSI) for the primary diagnosis of renal diseases on histology following the College of American Pathologists (CAP) guidelines.

The development of reliable artificial intelligence (AI) algorithms in pathology depends on solid ground truth provided by meticulous annotation of whole slide images (WSI), a time-consuming and operator-dependent process. A benchmark of the available annotation tools is performed to standardize and streamline this process.