University of Milano-Bicocca Showcase

Iterative Bleaching Extends Multiplexity (IBEX) immunofluorescence was performed on skin samples collected from CD11c-DOG mice either treated with diphtheria toxin (Skin_CD11cDOG_with_Diphtheria_Toxin) or phosphate-buffered saline (PBS) treated controls (Skin_CD11cDOG_no_Diphtheria_Toxin). For dendritic cell depletion, CD11c-DOG mice received three systemic intravenous injections of diphtheria toxin (16 ng/g body weight) on days −3, −2, and −1. Control mice PBS following the same schedule. In addition, a local subcutaneous injection of diphtheria toxin (16 ng) was administered on day −1 at the site designated for tissue collection. Mice were euthanized on day 0 in accordance with national and international regulations for animal care and use. A square section of flank skin was excised from each mouse at the site of the last intradermal injection. Subcutaneous adipose tissue was mechanically removed using forceps. Skin samples were subsequently processed according to the 2D Manual IBEX protocol for multiplex immunofluorescence imaging.

Live-cell holotomographic imaging of OHP-induced pathological changes in DRG neurons. These videos show primary adult mouse DRG neuronal cultures exposed to OHP at 7.5 or 25 µM. Refractive index-based imaging captures neurite fragmentation and degeneration, morphological features consistent with necroptosis, and autophagic vacuoles. Images were acquired using a 5 × 5 grid scan, with each grid measuring 90 × 90 µm, every 15 min for 28 h at 7 fps, from 24 to 52 h after OHP incubation.

This is a set of images taken on In2Sight devices coupled to PVC (polyvinylchloiride), implanted in mice. -- Animal implants. Each mouse (BALB/cAnNHsd, Envigo) received one In2Sight device, which was implanted subcutaneously in the inner part of the hind leg. Mice were anesthetized intraperitoneally with 100mg/kg ketamine and 4mg/kg xylazine. The operating field was prepared by hair trichotomy followed by appropriate disinfection with 70° ethanol or betadine. Two incisions were then made using a sterile scalpel. After positioning the device, a tight suture (single stitch) of the incision was performed using a sterile 5/0 absorbable suture thread. For the first 2 days, pain control was foreseen by administering opioids (Buprenorphine hydrochloride 0.16mg/kg subcutaneously) and infections avoided by administering an antibiotic (Enrofloxacin 5 mg/kg for 5 days). All the implants were done under the animal permit n. 551/2023-PR of the Italian ministry for health. The device, once coupled with acrylic glue to a slab of PVC (Polyvynil chloride, 5 mm in diameter, 0.3 mm thickness), was sterilized by STERRAD before the implant that lasted up to 6 weeks. The mice were kept in a pathogen‐free conventional animal house facility. The animal house is run by professional employees fully equipped with state‐of‐the‐art instrumentation in order to maintain the standard of animal welfare at the maximum levels. All mice were housed in individual, ventilated cages with 12 h light/dark cycles with food and water ad libitum. The mice were euthanized at the end of the implant by cervical dislocation right before the imaging session. Experiments were performed using protocols approved by the Institutional Animal Care and Use Committee of the University of Milano‐Bicocca and by the Italian Ministry of Health. -- Chemicals. Hoechst 33342 (Sigma-Aldrich Chemical Company (Steinheim, Germany)) was used to stain nuclei in ex-vivo experiments. -- Multiphoton imaging. Third harmonic generation and 3-photon excitation fluorescence were selected by the Semrock filters FF02-447/60 and FF562-Di03-25, respectively. For SHG the exc. wavelength was 800 nm (emission 400/40 nm). λexc was 770 nm in case of Hoechst nuclei staining (485/30 nm). Auto-fluorescence in the green channel was collected at 535/50 nm. FILE NAME FORMAT for folder TPE_SHG_6weeks: name@800P140mWBP400BP535z200um50piani_Ch2 is taken as an example. name: if "lente#" (#=1,..4) means one of the 4 lenses on top of the microatlas; if "spacer" means the In2Sight spacers; if "fralenti" means the space between the lenses and therefore in the free tissue 800P140m: excitation wavelength = 800 nm, average power = 140 mW, WBP400BP535z200um50piani: means emission filters at 400 nm (Ch1) and 535 nm (Ch2); 200 micrometer full depth of stack, 50 layers. FORMAT in THG_3PE_data_1week: modality, exc. , through the lenses or control, FOV in um, image size in pixels, the depth, the number of averages (#avg).

This archive contains the example datasets, implementations of the rational splines and P-spline methods, along with code to reproduce the benchmark and the generate the figures in the publication "Shape preserving interpolation with arbitrary smoothness based on mollification". Archive structure ----------------- - `interp.py`: implementation of the interpolation methods - `benchmark.py`: program that benchmarks the interpolation code - `plots.py`: program that generates the figures of the article - `proofs.py`: mathematical proofs in [SymPy][1] of some results used to implement the rational splines - `shell.nix`: [Nix][2] expression that builds a reproducible environment to run the programs

This database provides a validated set of sentences designed for research on ethnic stereotyping and social cognition. The materials are suitable for use in EEG/ERP experiments, neuroimaging studies (fMRI, MEG), behavioral paradigms, and computational/LLM modelling of social bias. The stimuli were validated on a native Italian-speaking population. The database consists of 330 sentence–word pairs organized across three experimental conditions: • Incongruent (N = 110): sentences in which the terminal target word violates the ethnic stereotype associated with the speaker's accent (e.g., a speaker with an African accent described as a pilot or a surgeon) • Congruent (N = 110): sentences in which the terminal word is consistent with prevailing ethnic stereotypes (e.g., a speaker with an African accent described as working in a field or selling items at a market stall) • Neutral (N = 110): control sentences mentioning Italian individuals or regional groups, with no ethnic accent or stereotype content Sentences are distributed across seven ethnic/accent categories: African, Chinese, Roma/Sinti, Eastern European, Asian, Latin American, and Arabian. All three conditions are matched on the following variables: • Sentence length in characters • Sentence length in words • Terminal word length in characters • Lexical frequency of the terminal word • Grammatical category and imageability of the terminal word • Gender of the protagonist (male / female / mixed) Intended uses • EEG/ERP studies of stereotyping, social prediction, and language processing (N400, LAN, LP components) • Neuroimaging (fMRI/MEG) studies of social cognition and predictive coding • Behavioral studies of implicit ethnic bias, semantic priming, and accent perception • Training, testing, or probing Large Language Models (LLMs) on social bias, world knowledge and stereotype content Availability of auditory stimuli A professionally recorded auditory version of these stimuli (sentences spoken with native-accented voices across the seven ethnic/accent categories) is also available. Auditory stimuli are not shared openly, but may be made available to research groups for non-profit academic purposes under a co-authorship agreement: groups wishing to use the auditory materials in a study intended for publication are invited to contact the authors to discuss the terms of scientific collaboration, which will include co-authorship on any resulting paper. To inquire, write to: mado.proverbio@unimib.it

Molecular Dynamics Simulation of T4-Lysozyme in the WT and L99A variant

Molecular Dynamics Simulation of od PDZ3 domina in the apo form and bound to an activator peptide

Image taken under SHG and THG at 1300 nm on IN2SIGHT devices coupled to PVC (Poly-vinyl-chloride) slabs implanted in mice. These data have been analyzed and used for the Ms. submitted on June 2026.