Contributors:Giorgio Cattoretti, Maddalena M Bolognesi, Francesca M Bosisio, Mario Faretta, Francesco Mascadri et al
Background: Antibody validation for tissue staining is required for reproducibility and criteria to ensure validity have been published recently. The majority of these recommendations imply the use of routinely processed tissue (FFPE).
Materials & Methods: We applied to lightly fixed frozen sections a panel of 126 antibodies validated for FFPE with extended criteria.
Results: Less than 30% performed conservatively with all fixations, 35% preferred one fixation over another, 13% gave non-specific staining, 23% did not stain at all.
Conclusions: Individual antibody variability of the paratope fitness for the fixed antigen may be the cause. Re-validation of established antibody panels is required when applied to sections whose fixation and processing is different from the tissue where they have been initially validated.
These are supplementary Data to the manuscript. In addition Tabel 1 and Supplementary Table 1 are provided in Excel format.
Structure of bombesin peptide calculated in presence of 150 mM SDS micelles at pH 7.0, 25°C.
Structure was determinded by NMR spectroscopy and SA-MD.
Data clearly show that, under these experimental conditions, bombesin adopts a α-helix structure.
Contributors:Elena Eva, Marco Giovanni Malusa, Stefano Solarino
Supporting Information for the article entitled " Seismotectonics at the transition between opposite-dipping slabs (western Alpine region)" written by E. Eva, M.G. Malusà and S. Solarino.
Contents of this file: Details on data processing; Distribution of P+S phase picks for the stations used in this study; Data selected for the comparison between LSQR and SVD performances; Vertical errors calculated using HypoDD with SVD; Focal mechanisms computed for this study; Dataset after HypoDD relocation.