Antigen-bearing proteins become progressively unavailable to immunodetection after prolonged storage of routine sections, exposed to a variety of agents, such as moisture, oxygen and temperature. By proteomic analysis, the antigens are retained in the sections and definitely in the tissue block, pointing to fixation-independent, storage time-dependent protein modifications. Based on previous experience, we hypothesized that a combined exposure to a reducing agent and to chemicals favouring protein conformation changes would reverse the masking in aged sections. Disaccharides, lactose and sucrose, and a surfactant, added to a standard antigen retrieval buffer, reverse the negative changes in aged sections. Furthermore, they provide enhanced access to antigens in freshly cut sections, but not universally, revealing additional factors, besides heat and calcium chelation, required for antigen retrieval of individual proteins. This dataset contains supplementary data to the manuscript, raw data and analytical tools.

Metabolite libraries built from NMR spectra for the metabolomics analysis of cinnamon extracts, using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 14.1 software.

This is folder for data and documents useful to draft the regulatory plan and to prepare all the actions related to WP5.

The data here provided have been used to analyse the structural evolution of the Arusan sector of the Great Kavir - Doruneh Fault system in Central Iran, one of the major intracontinental active strike-slip faults extending from the Afghan border to the Nain region between Central Iran and the Sanandaj-Sirjan Zone. We document important dextral shearing recorded along a segment of the GKDFS, the Arusan Fault System (AFS), located east of Jandaq, close to the present-day active trace of the GKDFS. The AFS include several ENE-WSW striking strands exposed for a length of more than 50 km, which couple pre-Cretaceous ophiolites and metamorphic basement units with the Cretaceous succession of the Khur basin. The fault shows transpressional structures consistent with a dextral shear including thrusts and en échelon folds affecting the Cretaceous carbonate units. Paleostress reconstruction estabilished vorticity parameters indicating that deformation occurred close to a total simple shear regime with a calculated Wk between 0.9 and 1. The enormous Meso-Cenozoic dextral displacements occurred along the AFS and along the entire GKDFS are attested by the up to several hundreds of kilometers offset of the Paleotethys suture zone, from NE Iran to the western border of Central Iran. We provide here the original field data, elaboration of satellie ASTER images for gelogical interpretations, geolocalization of each structural measurement sites that we used for analyses ad interpretations in our work. The data details are as follows: - FILE_1: Geological map of the Arusan Fault System with the localization of all measurement sites - FILE_2: A kml file with the geolocalization of measurement sites - FILE_3: Georeferenced false color composite image generated from the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) Level 1B data collected on 13 September 2003. RGB combination shows the principal components derived from the Gram-Schmidt spectral sharpening tool. - FILE_4: Field images of fault planes with kinematic indicators - FILE_5: Fault slip data used for paleostress calculations

Data related to the design of the microoptics for in2sight, D2.1

Dropen is a fully automatic drop image analysis software in order to study the wetting properties of solid surfaces. It also includes the manual selection and fitting approaches. This software is developed in MATLAB using GUIDE. Regarding the identification of contact points two approaches are provided in Dropen: 1. Automatic: determines the left and right contact points automatically using a precise image processing code; 2. Manual: let the user select the left and right contact points manually by pushing the “Manual selection” button. In the case of the wrong selection of contact points the software let the user try two more times without a need to push the “Manual selection” button again. Regarding the contact angle measurement, the software provides a fully- automatic convolution mask method and also fitting with the circle and polynomial equations. So, the contact angle measurement panel includes: 1. Mask method: the fully automatic contact angle measurement, 2. Fitting: includes: a. Circle b. Polynomial: user can change the polynomial order. Regarding our studies, the optimum polynomial order for superhydrophilic droplets is two and for the rest of the droplets is Three. So, it is recommended to do not to change the polynomial order. After choosing the contact point identification and contact angle measurement methods, push the RUN button to find the results in the Result panel. In addition, the magnified images of the left and right contact areas are presented there. The drop contour line and the contact points have been shown with blue and red markers on the image, respectively. It is possible to save the contact angle data, as well as the left and right contact area images by pushing the corresponding push buttons. This software is developed in SEFILAB at the Department of Materials science, University of Milano-Bicocca, and is available at the UNIMIB repository (http://dx.doi.org/10.17632/wzchzbm58p.3). This repository will be updated by the new modified versions of the software.

materials and methods as supplementary data to the review "Cannabinoids: an effective treatment for chemotherapy-induced peripheral neurotoxicity?"

Supplementary Tables and Figures are made available here.